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mouse anti human tlr3  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology mouse anti human tlr3
    AME-1 upregulates <t>TLR3</t> expression. (A) HMC3 cells were treated with AME-1 (50–5,000 μg/mL) for 3 h, RNA was extracted and analyzed via RT-qPCR. Results are presented as fold-gene expression relative to untreated cells and statistics were calculated using One-Way ANOVA with Dunnett’s multiple comparison post hoc analysis relative to fold-change in GAPDH expression: p ≤ 0.01 (**). Data presented as mean ± SEM for n = 3. HMC3 were treated with AME-1 (50–5,000 µg/mL), LPS (1 µg/m) or poly(I:C) (0.5 µg/mL) for 24 h, the cells were lysed and analyzed via Western blot. (B) TLR3 and IRF3 protein expression and (E, F) corresponding densitometry analysis. (C, D) MDA5 and RIG-I protein expression and (G, H) corresponding densitometry analysis. Densitometry analysis was performed on three blots on the indicated bands and statistical significance was calculated using one-way ANOVA with Tukey post hoc analysis.
    Mouse Anti Human Tlr3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human tlr3/product/Santa Cruz Biotechnology
    Average 94 stars, based on 213 article reviews
    mouse anti human tlr3 - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Amanita muscaria extract potentiates production of proinflammatory cytokines by dsRNA-activated human microglia"

    Article Title: Amanita muscaria extract potentiates production of proinflammatory cytokines by dsRNA-activated human microglia

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2023.1102465

    AME-1 upregulates TLR3 expression. (A) HMC3 cells were treated with AME-1 (50–5,000 μg/mL) for 3 h, RNA was extracted and analyzed via RT-qPCR. Results are presented as fold-gene expression relative to untreated cells and statistics were calculated using One-Way ANOVA with Dunnett’s multiple comparison post hoc analysis relative to fold-change in GAPDH expression: p ≤ 0.01 (**). Data presented as mean ± SEM for n = 3. HMC3 were treated with AME-1 (50–5,000 µg/mL), LPS (1 µg/m) or poly(I:C) (0.5 µg/mL) for 24 h, the cells were lysed and analyzed via Western blot. (B) TLR3 and IRF3 protein expression and (E, F) corresponding densitometry analysis. (C, D) MDA5 and RIG-I protein expression and (G, H) corresponding densitometry analysis. Densitometry analysis was performed on three blots on the indicated bands and statistical significance was calculated using one-way ANOVA with Tukey post hoc analysis.
    Figure Legend Snippet: AME-1 upregulates TLR3 expression. (A) HMC3 cells were treated with AME-1 (50–5,000 μg/mL) for 3 h, RNA was extracted and analyzed via RT-qPCR. Results are presented as fold-gene expression relative to untreated cells and statistics were calculated using One-Way ANOVA with Dunnett’s multiple comparison post hoc analysis relative to fold-change in GAPDH expression: p ≤ 0.01 (**). Data presented as mean ± SEM for n = 3. HMC3 were treated with AME-1 (50–5,000 µg/mL), LPS (1 µg/m) or poly(I:C) (0.5 µg/mL) for 24 h, the cells were lysed and analyzed via Western blot. (B) TLR3 and IRF3 protein expression and (E, F) corresponding densitometry analysis. (C, D) MDA5 and RIG-I protein expression and (G, H) corresponding densitometry analysis. Densitometry analysis was performed on three blots on the indicated bands and statistical significance was calculated using one-way ANOVA with Tukey post hoc analysis.

    Techniques Used: Expressing, Quantitative RT-PCR, Gene Expression, Comparison, Western Blot



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    AME-1 upregulates <t>TLR3</t> expression. (A) HMC3 cells were treated with AME-1 (50–5,000 μg/mL) for 3 h, RNA was extracted and analyzed via RT-qPCR. Results are presented as fold-gene expression relative to untreated cells and statistics were calculated using One-Way ANOVA with Dunnett’s multiple comparison post hoc analysis relative to fold-change in GAPDH expression: p ≤ 0.01 (**). Data presented as mean ± SEM for n = 3. HMC3 were treated with AME-1 (50–5,000 µg/mL), LPS (1 µg/m) or poly(I:C) (0.5 µg/mL) for 24 h, the cells were lysed and analyzed via Western blot. (B) TLR3 and IRF3 protein expression and (E, F) corresponding densitometry analysis. (C, D) MDA5 and RIG-I protein expression and (G, H) corresponding densitometry analysis. Densitometry analysis was performed on three blots on the indicated bands and statistical significance was calculated using one-way ANOVA with Tukey post hoc analysis.
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    Image Search Results


    AME-1 upregulates TLR3 expression. (A) HMC3 cells were treated with AME-1 (50–5,000 μg/mL) for 3 h, RNA was extracted and analyzed via RT-qPCR. Results are presented as fold-gene expression relative to untreated cells and statistics were calculated using One-Way ANOVA with Dunnett’s multiple comparison post hoc analysis relative to fold-change in GAPDH expression: p ≤ 0.01 (**). Data presented as mean ± SEM for n = 3. HMC3 were treated with AME-1 (50–5,000 µg/mL), LPS (1 µg/m) or poly(I:C) (0.5 µg/mL) for 24 h, the cells were lysed and analyzed via Western blot. (B) TLR3 and IRF3 protein expression and (E, F) corresponding densitometry analysis. (C, D) MDA5 and RIG-I protein expression and (G, H) corresponding densitometry analysis. Densitometry analysis was performed on three blots on the indicated bands and statistical significance was calculated using one-way ANOVA with Tukey post hoc analysis.

    Journal: Frontiers in Pharmacology

    Article Title: Amanita muscaria extract potentiates production of proinflammatory cytokines by dsRNA-activated human microglia

    doi: 10.3389/fphar.2023.1102465

    Figure Lengend Snippet: AME-1 upregulates TLR3 expression. (A) HMC3 cells were treated with AME-1 (50–5,000 μg/mL) for 3 h, RNA was extracted and analyzed via RT-qPCR. Results are presented as fold-gene expression relative to untreated cells and statistics were calculated using One-Way ANOVA with Dunnett’s multiple comparison post hoc analysis relative to fold-change in GAPDH expression: p ≤ 0.01 (**). Data presented as mean ± SEM for n = 3. HMC3 were treated with AME-1 (50–5,000 µg/mL), LPS (1 µg/m) or poly(I:C) (0.5 µg/mL) for 24 h, the cells were lysed and analyzed via Western blot. (B) TLR3 and IRF3 protein expression and (E, F) corresponding densitometry analysis. (C, D) MDA5 and RIG-I protein expression and (G, H) corresponding densitometry analysis. Densitometry analysis was performed on three blots on the indicated bands and statistical significance was calculated using one-way ANOVA with Tukey post hoc analysis.

    Article Snippet: The membrane was subsequently blocked for 1 h at room temperature with 5% skim milk in TBS-T followed by incubation for1hr with 0.4 μg/mL of mouse anti-human TLR3 (sc-32232, Santa Cruz Biotechnology, Dallas, TX, United States), 0.5 μg/mL of mouse anti-human IRF3 (550428, BD biosciences), 2.5 μg/mL of rabbit anti-human MDA5 (33H12L34, Invitrogen), 1 μg/mL rabbit anti-human RIG-1 (PA510297, Invitrogen), or 1 μg/mL of mouse anti-β-actin loading control (PA1-183, Invitrogen) in 3% BSA-TBS-T, and finally incubated for 1 h with goat anti-mouse IRDye 680 (Li-Cor, Lincoln, NE, United States) or goat anti-rabbit IRDye 800 (Li-Cor) diluted 1:15,000 in 3% BSA-TBS-T.

    Techniques: Expressing, Quantitative RT-PCR, Gene Expression, Comparison, Western Blot

    Antibodies used in the study for cell immunophenotyping.

    Journal: Scientific Reports

    Article Title: Impact of chronic HCV treatment on quality of life of patients with metabolic disorders in context of immunological disturbances

    doi: 10.1038/s41598-020-67296-9

    Figure Lengend Snippet: Antibodies used in the study for cell immunophenotyping.

    Article Snippet: mouse anti-human TLR3 , PE , TLR3.7 , Santa Cruz Biotechnology.

    Techniques:

    TLR3 expression level on T lymphocytes based on the mean fluorescence intensity; median levels before (T0) and after the treatment (T1) compared with the control group. All presented measurements differ significantly (Mann-Whitney U test and Wilcoxon signed-rank test, p < 0.05).

    Journal: Scientific Reports

    Article Title: Impact of chronic HCV treatment on quality of life of patients with metabolic disorders in context of immunological disturbances

    doi: 10.1038/s41598-020-67296-9

    Figure Lengend Snippet: TLR3 expression level on T lymphocytes based on the mean fluorescence intensity; median levels before (T0) and after the treatment (T1) compared with the control group. All presented measurements differ significantly (Mann-Whitney U test and Wilcoxon signed-rank test, p < 0.05).

    Article Snippet: mouse anti-human TLR3 , PE , TLR3.7 , Santa Cruz Biotechnology.

    Techniques: Expressing, Fluorescence, MANN-WHITNEY

    Percentage of T lymphocytes with TLR3 expression before (T0) and after (T1) the treatment. The presented measurements differ significantly (Wilcoxon signed-rank test, p < 0.05).

    Journal: Scientific Reports

    Article Title: Impact of chronic HCV treatment on quality of life of patients with metabolic disorders in context of immunological disturbances

    doi: 10.1038/s41598-020-67296-9

    Figure Lengend Snippet: Percentage of T lymphocytes with TLR3 expression before (T0) and after (T1) the treatment. The presented measurements differ significantly (Wilcoxon signed-rank test, p < 0.05).

    Article Snippet: mouse anti-human TLR3 , PE , TLR3.7 , Santa Cruz Biotechnology.

    Techniques: Expressing